If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292) This work has been supported in part by the Flow Cytometry Core at the H. The FCC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for all Core requests, scheduling, billing, and usage tracking.Īll publications based on work conducted in the Flow Cytometry Core Facility should acknowledge the facility. The Flow Cytometry Core is located in the Moffitt Research Center (MRC) on the first floor, room 1295. Usage is on a first-come, first-served basis however, priority is given to Moffitt Cancer Center members with peer-reviewed funded projects. After hours, unassisted usage of the analyzers is available for trained and approved users. Assisted after-hours usage must be scheduled in advance and approved by the Core Facility Manager. Core Staff are available for assisted services Monday-Friday, 9 a.m.-6 p.m. If you have only 1 data point, you cant draw any conclusion since what you are. The Flow Cytometry Core is open 24 hours a day, seven days a week. Flow Cytometry: Designing multi-colour panels, machine compensation (FACS Canto, LSR II, MacsQuant), sample acquistion, cell sorting of rare populations and. In general biological replicates are required to do statistical analysis. Current literature base including cytometry journals, manufacturer information on instrumentation, methodology, protocols, techniques, and the latest vendor products and services.Instrument training and project consultation.Multi-parameter analysis including DNA cell cycle, ploidy, apoptosis, viability, phenotyping, kinetics, cytokines, fluorescent proteins, proliferation, signal transduction, bead array, and morphology.The services that are provided by this Core include: (1) multi-parameter single-cell analyses, including cell cycle, apoptosis, viability, cell proliferation, ploidy, phenotyping, fluorescent proteins, signal transduction, bead array kinetics, cytokines, exosomes, and morphology (2) cell sorting (3) courses and workshops (4) instrument training and project consultation and 5) experiment and panel design. Brian Ruffell, the Flow Cytometry Core provides Moffitt members with access to state-of-the-art instrumentation and cutting-edge flow cytometry applications.
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